ASO-Checker is an accompanying tool to MASON which can be used to check different attributes of a pre-selected ASO. The tool takes ASO sequences and checks for several important sequence- and target-species related attributes. Similar to the MASON algorithm, it screens for off-targets and evaluates whether the sequence is suitable for further use.
Please select a unique custom ID to store the result
Select one of the preset genomes or use "Own files" to upload your own genome.
Select the preset genomes of Salmonella (substr. SL1344) (FASTA, GFF), E. coli (K12) (FASTA, GFF), Clostridium difficile 630 (FASTA, GFF) and Fusobacterium nucleatum (ATCC 23726) (FASTA, GFF). Other genomes (FASTA) and their annotations (GFF) can be downloaded from the NCBI website and uploaded using the "Own files" option. For more details on how to download custom genomes, have a look at our help page
Below, custom FASTA and GFF files of a bacterium of interest can be uploaded (can be downloaded from NCBI):
Please add ASO-sequences (ATGC-alphabet) of the ASOs that you want to be checked below. Use FASTA format if you want to add more than one sequence (max. 10 sequences allowed, length 8-14 bases):
