MASON (Make AntiSense Oligomers Now) is a web-tool which guides the design of bacterial antisense oligonucleotides (ASOs). We initially developed it for peptide nucleic acids (PNAs), although it can also be used for designing other ASOs such as PMOs or LNAs. All you need is a genome and the gene you want to target.
Read our paper: RNA Journal - Design and off-target prediction for antisense oligomers targeting bacterial mRNAs with the MASON webserver public
Report bugs/problems by opening a GitHub issue: github.com/BarquistLab/mason/issuesbug_report
Contact us via email: jakobjung@tutanota.com email
Start designing ASOs for targeting any bacterial gene: start MASON fast_forward
MASON update - 2024-05
We have updated the MASON webserver with two new features: ASO-checker and Scrambler. ASO-checker validates your ASO design by checking for off-targets in the genome as while checking for different ASO-specific features. Scrambler scrambles the sequence of your ASO to generate the best negative control ASO sequence for your experiment. Therefore, there are now three options available on the MASON homepage:
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